Description
This assay is a quantitative sandwich ELISA. The microtiter strips are pre-coated with a polyclonal antibody specific for mouse PR3. Standards and samples are pipetted into the wells and any mouse PR3 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidise (HRP) labelled polyclonal antibody specific for mouse PR3 is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of mouse PR3 bound initially. The assay is stopped and the optical density of the wells is determined using a micro-plate reader. Since the increase in absorbance is directly proportional to the amount of captured mouse PR3, the unknown sample concentration can be interpolated from a reference curve included in each assay